This web page contains a live and functional spreadsheet created with
SpreadsheetConverter
. Please enable JavaScript in your web browser for the live calculations to work properly.
Enter your desired concentration factors. This will update the Stock Concentration values and adjust the aliquot chart below. After completing this form, print or save your results for an easy reference.
Macro and Micronutrients Needed
Stock solution
Component
Final Conc. (mg/L)
Stock Conc. (mg/L)
Stock Sln. (g/100ml)
Concentration Factor
A
Na
2
.EDTA
37.3
"0"
FeSO
4
. 7H
2
O
27.8
"0"
B
(NH
4
)SO
4
134
"0"
C
CoCl
2
.6H
2
O
0.025
"0"
KI
0.75
"0"
D
KNO
3
2500
"0"
E
NaH
2
PO
4
130.5
"0"
ZnSO
4
· 7H
2
O
2
"0"
CuSO
4
. 5H
2
O
0.025
"0"
MgSO
4
122.09
"0"
F
MnSO
4
. H
2
O
10
"0"
H
3
BO
3
3
"0"
Na
2
MoO
4
. 2H
2
O
0.25
"0"
G
CaCl
2
113.240
"0"
Vitamins Needed
Stock solution
Component
Final Conc. (mg/L)
Stock Conc.(mg/L)
Stock Sln. (g/100ml)
Concentration Factor
H
Thiamine
10
"0"
"0"
Nicotinic Acid
1
"0"
"0"
Myo-Inositol
100
"0"
"0"
Pyridoxine
1
"0"
"0"
pH before autoclaving
5.8
Gamborg Media Solution Planner
Desired total preparation volume
mL
Enter your desired total preparation volume. Use the chart below to determine appropriate aliquots
of each component to use. Follow the protocol to add each stock solution in the appropriae order.
Macro and Micronutrients Needed
Stock solution
Component
Stock Conc. (mg/L)
Aliquot (ml)
Final Conc. (mg/L)
A
Na
2
.EDTA
"0"
37.3
FeSO
4
. 7H
2
O
"0"
27.8
B
(NH
4
)SO
4
"0"
134
C
CoCl
2
.6H
2
O
"0"
0.025
KI
"0"
0.75
D
KNO
3
2500
E
NaH
2
PO
4
"0"
130.5
ZnSO
4
· 7H
2
O
"0"
2
CuSO
4
. 5H
2
O
"0"
0.025
MgSO
4
"0"
122.09
F
MnSO
4
. H
2
O
"0"
10
H
3
BO
3
"0"
3
Na
2
MoO
4
. 2H
2
O
"0"
0.25
G
CaCl
2
"0"
113.240
Vitamins Needed
Stock solution
Component
Stock solution concentration (mg/L)
Aliquot (ml)
Final Concentration (mg/L)
H
Thiamine
"0"
""
10
Nicotinic Acid
"0"
1
Myo-Inositol
"0"
100
Pyridoxine
"0"
1
pH before autoclaving
5.8
Reset
Print