| |
Enter your desired concentration factors. This will
update the Stock Concentration values and adjust the aliquot chart below.
After completing this form, print or save your results for an easy reference.
|
|
|
|
| |
|
| |
|
|
|
|
|
|
|
| |
Macro and Micronutrients Needed
|
|
|
| |
Stock solution
|
Component
|
Final Conc. (mg/L)
|
Stock Conc. (mg/L)
|
Stock Sln. (g/100ml)
|
Concentration Factor
|
|
| |
|
| |
A
|
Na2.EDTA
|
37.3
|
|
|
|
|
| |
|
|
|
|
|
|
| |
FeSO4. 7H2O
|
27.8
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
B
|
(NH4)NO4
|
400
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
C
|
Ca(NO3)2
|
386
|
|
|
|
|
| |
|
|
|
|
|
|
| |
ZnSO4 · 7H2O
|
8.6
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
D
|
CuSO4. 5H2O
|
0.25
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
E
|
MgSO4
|
180.7
|
|
|
|
|
| |
MnSO4. H2O
|
22.3
|
|
|
|
|
| |
|
|
|
|
|
|
| |
K2SO4
|
990
|
|
|
|
|
| |
H3BO3
|
6.2
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
F
|
KH2PO4
|
170
|
|
|
|
|
|
|
| |
Na2MoO4.2H2O
|
0.25
|
|
|
|
|
|
|
| |
CaCl2
|
72.5
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| |
Vitamins Needed
|
|
| |
Stock solution
|
Component
|
Final Conc. (mg/L)
|
Stock
Conc.(mg/L)
|
Stock Sln.
(g/100ml)
|
Concentration Factor
|
|
| |
|
| |
G
|
Glycine
|
2
|
|
|
|
|
| |
Myo-Inositol
|
100
|
|
|
|
|
| |
Nicotinic Acid
|
0.5
|
|
|
|
|
| |
Pyridoxine
|
0.5
|
|
|
|
|
| |
Thiamine
|
1
|
|
|
|
|
| |
pH before autoclaving
|
5.8
|
|
|
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| |
McCown-Woody Media Solution Planner
|
|
|
| |
|
|
|
|
|
|
|
| |
Desired total preparation volume
|
|
mL
|
|
|
|
Enter your
desired total preparation volume. Use the chart below to determine
appropriate aliquots
of each component to use. Follow the protocol to add each stock solution in
the appropriae order.
|
|
| |
|
| |
|
|
|
|
|
|
|
| |
Macro and Micronutrients Needed
|
|
|
| |
Stock solution
|
Component
|
Stock Conc. (mg/L)
|
Aliquot (ml)
|
Final Conc. (mg/L)
|
|
|
| |
|
|
| |
A
|
Na2.EDTA
|
|
|
37.3
|
|
|
| |
FeSO4. 7H2O
|
|
27.8
|
|
|
|
|
|
|
|
|
|
|
| |
B
|
(NH4)NO4
|
|
|
400
|
|
|
|
|
|
|
|
|
|
|
| |
C
|
Ca(NO3)2
|
|
|
386
|
|
|
| |
ZnSO4 · 7H2O
|
|
8.6
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
D
|
CuSO4. 5H2O
|
|
|
0.25
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
E
|
MgSO4
|
|
|
180.7
|
|
|
| |
MnSO4. H2O
|
|
22.3
|
|
|
| |
K2SO4
|
|
990
|
|
|
| |
H3BO3
|
|
6.2
|
|
|
|
|
|
|
|
|
|
|
|
|
| |
F
|
KH2PO4
|
|
|
170
|
|
|
|
|
| |
Na2MoO4.2H2O
|
|
0.25
|
|
|
| |
CaCl2
|
|
72.500
|
|
|
|
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| |
Vitamins Needed
|
|
|
| |
Stock solution
|
Component
|
Stock solution concentration (mg/L)
|
Aliquot (ml)
|
Final Concentration (mg/L)
|
|
|
| |
|
|
| |
|
|
| |
G
|
Glycine
|
|
|
2
|
|
|
| |
Myo-Inositol
|
|
100
|
|
|
| |
Nicotinic Acid
|
|
0.5
|
|
|
| |
Pyridoxine
|
|
0.5
|
|
|
| |
Thiamine
|
|
1
|
|
|
| |
pH before autoclaving
|
5.8
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
|